03 February 2020 | News
GenScript’s R&D team developed a qRT PCR detection assay which has successfully detected 2019-nCoV in positive control samples RdRP gene, N gene and E gene
image courtesy: marketwatch
The 2019 Novel Coronavirus (2019-nCoV) was first detected in Wuhan, China in December 2019. Since the first cases were reported, the outbreak has rapidly spread through China and now several other countries worldwide. As of January 29, 2020, there are 6,161 confirmed cases, 132 deaths in 19 countries, including the United States, France, and Japan.
The 2019-nCoV has been reported as difficult to detect during the first two weeks after initial infection. Infected individuals may not be aware of their contagiousness, thus putting others at risk of contracting the virus. There are cases reported that people can be infectious without showing symptoms. A fast and reliable detection method can help researchers better understand the biology of the disease and potentially guide future diagnostics and treatment
To help expedite 2019-nCoV research, GenScript’s R&D team developed a qRT PCR detection assay based on GenBank Sequence NC_045512.2. This assay has successfully detected 2019-nCoV in positive control samples RdRP gene, N gene and E gene. Additional testing is ongoing and this assay should be considered for research use only (RUO).
Disclaimers from GenScript:
GenScript also provides plasmids containing parts of 2019-nCoV RdRP gene, N gene, E gene that can be used as positive controls for 2019-nCoV detection.