Thursday, 20 June 2019

Scientists bioengineer human liver disease in the lab

31 May 2019 | News

3D Human Liver Organoids help reveal molecular secrets of deadly disorder

Scientists successfully bioengineered human liver organoids that faithfully mimic key features of fatal liver disease in the laboratory. This allowed them to uncover underlying disease biology in the organoids and test a potential therapy that in preclinical lab tests reversed an often-fatal childhood condition called Wolman disease.

Researchers at Cincinnati Children's Hospital Medical Center say their study overcomes major hurdles to unraveling the molecular mysteries of liver diseases and finding desperately needed new therapies. It also leads to personalized methods to study inflammation and fibrosis in liver disease that match the unique genetics and biology of individual patients, according to study authors.

"Although current human organoid systems can recreate organ architecture in living lab organisms like mice, they fail to capture the complex pathologies of inflammation and fibrosis in liver diseases," said Takanori Takebe, MD, lead study investigator and a physician at the Cincinnati Children's Center for Stem Cell and Organoid Medicine (CuSTOM). 

"We developed a reproducible method to bioengineer complex, multicellular human liver organoids using pluripotent stem cells derived from healthy human donors and those with liver disease," he said. "These organoids generate different types of liver cells like hepatocytes, stellate and Kupffer-like cells, and they accurately recreate in the lab inflammation, fibrosis and other features of liver disease.''

That is significant because years of attempted drug development for conditions like fatty liver disease and Wolman have been hindered by a lack of human disease models that accurately mimic the diseases, Takebe said. And although certain animal models like mice are available, they aren't always reliable for studying human disease.

The study is a collaboration of scientists at Cincinnati Children's, the Institute of Research at Tokyo Medical and Dental University (TMDU) in Japan (where Takebe is also a member of the medical staff), and researchers at four other institutions in Japan and the United States.   

In this study, Takebe and colleagues used 11 different lines of healthy and diseased pluripotent stem cells to generate complex multi-cellular human liver organoids. The diseased pluripotent cells included those grown from donors with a deficiency in the LAL protein.

The researchers treated the organoids with free fatty acids—byproducts of fat metabolism in fatty tissue. This helped drive liver fat accumulation, inflammation and fibrosis. The organoids were able to recreate liver disease in the lab in a step-by-step, successive manner similar to Wolman disease in children.

As liver disease developed in the organoids, researchers used an extremely high-resolution imaging technology called atomic force microscopy. This allowed them to demonstrate and observe progressive levels of organ stiffening that they said confirms the increasing severity of accumulating liver fibrosis.

After concluding that Wolman disease in the human organoids was caused by LAL deficiency, the researchers were able to reverse that deficiency by exposing the organoids to a recombinant LAL protein. Next, study authors leveraged earlier research that shows LAL deficiency causes over activation of a protein called mammalian target of rapamycin (mTOR), which is linked to liver fibrosis.

Previous research also shows that mTOR can be suppressed by a receptor protein called farnesoid X receptor (FXR), which functions in the nucleus of intestinal and liver cells. FXR upregulates and down-regulates genes that control fat metabolism and bile acid processes in the liver. Takebe and his colleagues decided to treat the human liver organoids with Wolman disease with an FXR agonist called obeticholic acid. FXR is an acid that synthesizes a protein called fibroblast growth factor 19 (FGF19) in the ileum of the small intestine.

FXR treatment suppressed fat accumulation in the human liver organoids and prolonged their survival. This opens the possibility of further preclinical testing to see whether or not FXR treatment might be an appropriate strategy for treating Wolman disease, according to study investigators. Because the study's findings are preclinical and involve laboratory models, additional research is needed to show whether the findings will apply to clinical treatment in patients.

Funding support came from the Cincinnati Children's Research Foundation, a grant from JST PRESTO (Japan Science and Technology Agency) and the Mirai Program (JPMJMI18CB), a Public Health Service grant (P30DK078392), the Takeda Science Foundation, the Japan Agency for Medical Research and Development (JP18fk0210037h0001, JP18bm0704025h0001, JP18H02800) and the National Institutes of Health (UG3DK119982). Takebe is a New York Stem Cell Foundation-Robertson Investigator.

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