However, conventional manufacturing approaches face significant hurdles: plasmid-based transfection is often inefficient due to DNA-induced toxicity, while viral vectors carry inherent safety concerns and regulatory complexity. These limitations frequently necessitate multiple expansion cycles, making the process labor-intensive, costly, and prone to T-cell exhaustion.
Minicircle DNA offers a powerful alternative. With its minimal size and lack of bacterial backbone, minicircle DNA dramatically reduces DNA toxicity and immunogenicity—resulting in more efficient gene transfer and enhanced T-cell viability. When paired with transposon systems such as Sleeping Beauty or PiggyBac, it enables non-viral gene integration with near-random insertion profiles, significantly improving the safety profile of engineered CAR-T cells. These advantages also extend to the reprogramming of sensitive cell types, including stem cells.
Key Advantages of Minicircle DNA for CAR-T Cell Applications:
Accelerate your CAR-T cell development with minicircle DNA.
We're here to support your innovation.
https://www.plasmidfactory.com/custom-dna/minicircle-dna/
Find out more about successful Minicircle applications at our ESGCT Lunchtime Symposium October 08 | 01:30 pm - 02:30pm | more info to follow
https://www.esgctcongress.com/platinum-sponsors/plasmidfactory
